mouse anti myospheroid  (Developmental Studies Hybridoma Bank)

Journal: PLoS ONE

Article Title: The Rho-Family GTPase Rac1 Regulates Integrin Localization in Drosophila Immunosurveillance Cells

doi: 10.1371/journal.pone.0019504

Figure Lengend Snippet: (A) Lamellocytes were bled from at least 24 control ( w 1118 ), homozygous Rac1 J11 , and homozygous Rac2 Δ larvae approximately 40 hours post-parasitization and stained for Myospheroid protein expression (red), as well as actin (green). (B) Anti-Mys antibody was bound to the surface of lamellocytes bled from control ( w 1118 ) larvae, followed by internalization for various times as indicated (n = 12 larvae/time-point). The cells were not permeabilized (C) Graph indicating percentage of surface Myospheroid expressed on control ( w 1118 ) haemocytes. Fluorescent intensity was measured using ImageJ to calculate the amount of cell surface Myospheroid on lamellocytes. Asterisk indicates significant difference (One-way Anova, P <0.01), errors bars indicate ± SE; n = 12 larvae/time-point, 0 minutes n = 1256 haemocytes, 30 minutes n = 1294, 60 minutes n = 1175). (D) Anti-Mys antibody was bound to the surface of lamellocytes bled from control ( w 1118 ) larvae, followed by internalization for 120 minutes as indicated (n = 12 larvae/time-point). Cells were permeabilized to allow for visualization of internalized Myospheroid. Size bars always indicate 20 µm.

Article Snippet: Mouse monoclonal anti-Myospheroid (Developmental Studies Hybridoma Bank, Iowa, USA), lamellocyte-specific mouse monoclonal antibody (L1a) and plasmatocyte-specific monoclonal mouse anti-Nimrod , were all used undiluted, mouse monoclonal anti-Rac1 (BD Biosciences) diluted 1∶250, mouse monoclonal antibody anti-α-Tubulin (Sigma) diluted 1∶1,000, rabbit polyclonal anti-α-Tubulin (Abcam) diluted 1∶500, rat monoclonal anti-Hsp90 (Abcam) diluted 1∶300.

Techniques: Staining, Expressing

Journal: PLoS ONE

Article Title: The Rho-Family GTPase Rac1 Regulates Integrin Localization in Drosophila Immunosurveillance Cells

doi: 10.1371/journal.pone.0019504

Figure Lengend Snippet: (A) Control ( w 1118 ) and homozygous Rac1 J11 larvae were raised at 22°C or 29°C, lamellocytes were recovered from at least 12 larvae during three experiments approximately 40 hours post-parasitization and stained for Myospheroid protein expression (green), as well as α-Tubulin (red). Arrowheads indicate Myospheroid protein localization. Size bar indicates 20 µm. (B) Lamellocytes were recovered from at least 24 non-parasitized late third instar larvae and stained for Myospheroid protein expression (green), as well as α-Tubulin (red). Size bar indicates 20 µm (C) Anti-Mys antibody was bound to the surface of lamellocytes bled from either non-parasitized or parasitized control ( w 1118 ), homozygous Rac1 J11 , or homozygous UAS-Rac1;He-GAL4,Rac1 j11 larvae raised at the indicated temperature, followed by internalization for 30 minutes (n = 12 larvae/time-point).

Article Snippet: Mouse monoclonal anti-Myospheroid (Developmental Studies Hybridoma Bank, Iowa, USA), lamellocyte-specific mouse monoclonal antibody (L1a) and plasmatocyte-specific monoclonal mouse anti-Nimrod , were all used undiluted, mouse monoclonal anti-Rac1 (BD Biosciences) diluted 1∶250, mouse monoclonal antibody anti-α-Tubulin (Sigma) diluted 1∶1,000, rabbit polyclonal anti-α-Tubulin (Abcam) diluted 1∶500, rat monoclonal anti-Hsp90 (Abcam) diluted 1∶300.

Techniques: Staining, Expressing

Journal: PLoS ONE

Article Title: The Rho-Family GTPase Rac1 Regulates Integrin Localization in Drosophila Immunosurveillance Cells

doi: 10.1371/journal.pone.0019504

Figure Lengend Snippet: (A) Control ( w 1118 ), homozygous Rac1 J11 , homozygous Rac1 J11 ,P(Hsp83+) larvae were raised at 22°C, lamellocytes were recovered from at least 24 larvae approximately 40 hours post-parasitization and stained for Myospheroid protein expression (green), as well as α-Tubulin (red). Size bar indicates 20 µm. (B) Control ( w 1118 ), homozygous Rac1 J11 , homozygous Rac1 J11 ,P(Hsp83+) , and mys nj42 ;Rac1 j11 ,P(Hsp83) larvae were raised at 22°C and an encapsulation assay in response to parasitization by L. boulardi G486 was performed. Values for proper encapsulation were calculated by the following equation [(Number of properly encapsulated wasp eggs/number of parasitized larvae) x 100]. Numbers above the bars indicate the number of wasp-parasitized larvae examined.

Article Snippet: Mouse monoclonal anti-Myospheroid (Developmental Studies Hybridoma Bank, Iowa, USA), lamellocyte-specific mouse monoclonal antibody (L1a) and plasmatocyte-specific monoclonal mouse anti-Nimrod , were all used undiluted, mouse monoclonal anti-Rac1 (BD Biosciences) diluted 1∶250, mouse monoclonal antibody anti-α-Tubulin (Sigma) diluted 1∶1,000, rabbit polyclonal anti-α-Tubulin (Abcam) diluted 1∶500, rat monoclonal anti-Hsp90 (Abcam) diluted 1∶300.

Techniques: Staining, Expressing